Applications
Two primary applications are Cell Rupture and Homogenisation.
Cell Rupture
SFP’s general experience
in this field of application is that a single stage homogenisation process is adequate and that the style of the valve makes minimal
difference to the effectiveness of the disruption. It is our observation that as percentage cell content increases in the suspension
that the disruption yield is likely to drop.
Soft Mammalian Cells (i.e. Lymphocytes)........................ 1 to 15 MPa
Organelles & Mitrochrondia ...........................................
1 to 15 MPa
Parasites (Typanosomes).............................................. 1 to 15 MPa
Sperm ..........................................................................
1 to 35 MPa
Nematodes .....................................................................
10 to 35 MPa
E.coli ...............................................................................
30 to 140 MPa
Mammalian Tissue ......................................................... 70 to 150 MPa
Vegetative Cells and Cyanobacteria ................... 35 to 150 MPa
Yeast ...............................................................................
100 to 250 MPa
Stratum Corneum ........................................................... 140 to 200 MPa
DNA.................................................................................
100 to 250 MPa
Hard Bacteria (staphylococcus, streptococcus)............ 150 to 320 MPa
Homogenisation
Liposomes......................................................................
20 to 100 MPa
Microemulsions and nanoparticles................................ 20 to 350 MPa
Deagglomeration............................................................
30 to 350 MPa
Lipid Nanoparticles
50 to 200 MPa *
Microbial inactivation food preservation 200 to 400 MPa
* Temperatures to 80°C (lipid melt temperature) may be required, for some applicatiions
